Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

Table 2

PCR oligonucleotide primers used for RFLP analysis in the five candidate genes studied in gilthead seabream.

Amplified fragment		Primers
Fragment	Length (bp)	Name	Position	Sequence (5′-3′)	Tm (°C)
GH_A	1045–1900	SG1 [20]	Exon 1	AGAACCTGAACCAGACATGG	52.8
		R1R1 [20]	Intron 2	CTGCTGCCAGAGAATTACTG	54.1
GH_B	1172	GH_4F	Exon 4	GTTCTCTGTCTGGCGGTTCT	56.3
		GH_4R	3′UTR	AGCAACTGGGTCTAATGAATGT	54.4
IGF-I_A	944/954	IGFI_PF	Promoter	TCATCAGATGTCATTTGCAGAC	52.9
		IGFI_PR	Exon 1	TGCCACTGAAAGGAAAGAGC	56.8
IGF-I_B	1506	IGFI-1F	Exon 1	AGCGCTCTTTCCTTTCAGTG	56.8
		IGFI-1R	Exon 2	GCCTCTCTCTCCACACACAA	55.1
MSTN1_A	934	MSTN_1F	Promoter	GCCTGTCAGTGTGGGACTTT	55.0
		MSTN_1R	Exon 2	ACGATTCGATTGGCTTGAAT	57.4
MSTN1_B	881/886	MSTN_2F	Exon 2	AACAAGTGTTGAGCGTGTGG	55.5
		MSTN_2R	Exon 3	CTCCGAGATCTTCACCTCCA	57.2
PRL_A	1014–1063	PRL_PF	Promoter	GACTTTAACATGACCTGGAGGA	54.4
		PRL_PR	Exon 1	TGGTTTCTCTGTGAGCCATCT	57.7
PRL_B	864/765	PRL_1F	Exon 1	ATGGCTCACAGAGAAACCAA	54.0
		PRL_1R	Exon 2	GAGTTGTGCTGAGGGAGTGC	55.7
PRL_C	1549/1496	PRL_3-4F	Exon 3	GTAGGCTGGACGATGATGC	54.8
		PRL_3-4R	Exon 5	AGCAGGACAACAGGAAATGG	56.7
SL_A	1650	SL_PF	Promoter	GAACAGTGGTAATGACAGTCTCAA	53.9
		SL_PR	Exon 2	GTGAGCAGATAGGGCCAGAG	56.2
SL_B	704–721	SL_4F	Exon 4	CATTCTGTGCTGATGCTGGT	55.9
		SL_4R	Exon 5	GGGCATCTTTCTTGAAGCAG	56.1

N.P.: nonpolymorphic; GH: Growth Hormone; IGF-1: Insulin-Like Growth Factor I; MSTN: Myostatin; PRL: Prolactin; SL: Somatolactin. A, B, and C fragments defined by the primer positions.