Monocytes Do Not Transdifferentiate into Proper Osteoblasts

Figure 2

Differentiated monocytes (Mo), MOMPs (M), and PCMOs (P) express CD68. Representative picture of immunohistochemistry against CD68 in Mo, M, and P (𝑁=3, 𝑛=3). After treatment to obtain MOMPs and PCMOs (“day 6;” first column) all cell types are positive for CD68. 20 days after the differentiation—either with vitamin D (column 2) or dexamethasone (column 3)—cells also expressed CD68, while primary osteoblasts proved to be negative for CD68. Magnification 200x. Following differentiation with vitamin D, high numbers of giant cells (marked with →) appear. Macrophage-like cells are marked with ⇒.

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