Monocytes Do Not Transdifferentiate into Proper Osteoblasts
Figure 4
Differentiation treatment did not change bone marker expression. Expression pattern on important osteoblast marker gens detected by RT-PCR. Freshly isolated monocytes (Mo); M and P after treatment to obtain MOMPs and PCMOs and untreated control cells (Mo, M, P “day 6”), after differentiation with medium containing vitamin D (Mo, M, P “day 20 Vit. D”), or with medium containing dexamethasone (Mo, M, P “day 20 Dexa”); controls: macrophages (Ma), osteoclasts (Oc), primary osteoblasts (O), mesenchymal stem cells (MSC), nonadherent bone marrow cells (naBMC), and negative control (N). (, ).
