FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds

Figure 2

PCR verification of the vfFAD2-FDMV2A-vfDGAT2/pCAMBIA1301 recombinant vectors and A. fumigatus/vfFAD2-FDMV2A-vfDGAT2 transformant. (a)PCR verification of the vfFAD2-FDMV2A-vfDGAT2/pCAMBIA1301 recombinant vector. (b) PCR amplification of the A. fumigatus transformant using special primers. (c–f) A. fumigatus transformants grew in PDA medium containing 100 μg/mL hygromycin B. 1, vfDGAT2; 2: vfFAD2; 3: vfFAD2-FMDV2A-vfDGAT2; 4: pCAMBIA1301 vector; 5: DNA Marker. (c) A. fumigatus/vfFAD2-FDMV2A-vfDGAT2; (d)A.  fumigatus/vfFAD2: (e) A. fumigatus/vfDGAT2; (f) A. fumigatus.