Autophagic Cell Death Is Induced by Acetone and Ethyl Acetate Extracts from Eupatorium odoratum In Vitro: Effects on MCF-7 and Vero Cell Lines

Figure 3

DNA laddering assay showing no DNA fragmentation or necrosis in MCF-7 cells treated with EOace and EOea at various time intervals., Lanes 1: DNA 100 bp ladder; 2: positive control; 3: negative control (untreated cells); 4 and 5: EOea-treated MCF-7 cells—24 hours; 6 and 7: EOace-treated MCF-7 cells—24 hours; 8 and 9: EOea-treated MCF-7 cells—48 hours; 10 and 11: EOace-treated MCF-7 cells—48 hours; 12 and 13: EOea-treated MCF-7 cells—72 hours; 14 and 15: EOace-treated MCF-7 cells—72 hours.