Selection of the In Vitro Culture Media Influences mRNA Expression of Hedgehog Genes, Il-6, and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos

Table 1

Sequences of oligonucleotides and annealing temperature used in reverse transcription and nested-PCR.

mRNA	Nested PCR	Annealing temperature (^°C)	Size of the fragment (bp)	3^′-/5^′-end	Sequence of oligonucleotide 5^′→3^′
β-actin X03672		54	407	5^′	CAA GGT GTG ATG GTG GGA ATG G
				3^′	CAG GAT GGC GTG AGG GAG AGC A
shh NM_009170	Outer pair	55	530	5^′	GAG ACC CAA CTC CGA TGT GT
				3^′	GAT GTC GGG GTT GTA ATT GG
	Inner pair	56	150	5^′	CTG GCC AGA TGT TTT CTG GT
				3^′	CTC GGC TAC GTT GGG AAT AA
ihh NM_010544	Outer pair	58	438	5^′	CAC TTG TGG TGG AGA TGT G
				3^′	TAC CAC ACG CTT GTC AGC TC
	Inner pair	53	106	5^′	CTC TAA CCA CTG CCC TCC TG
				3^′	AGA GGA CGG AGA CAA CCT CA
il-6 NM_031168	Outer pair	56	339	5^′	GTT CTC TGG GAA ATC GTG GA
				3^′	GGA AAT TGG GGT AGG AAG GA
	Inner pair	56	226	5^′	TGT GCA ATG GCA ATT CTG AT
				3^′	CTC TGA AGG ACT CTG GCT TTG
nox NM_172203	Outer pair	59	683	5^′	GGG ATG ACC ATA AGG GGA GT
				3^′	CCA GCC AGT GAG GAA GAG AC
	Inner pair	59	216	5^′	TCC ATT TCC TTC CTG GAG TG
				3^′	CCC AAC CAG TAC AGC CAC TT
gpx4 NM_001037741	Outer pair	58	410	5^′	AGT ACA GGG GTT TCG TGT GC
				3^′	CGG CAG GTC CTT CTC TAT CA
	Inner pair	57	166	5^′	CCG GCT ACA ACG TCA AGT TT
				3^′	ACG CAG CCG TTC TTA TCA AT
gpx1 NM_008160	Outer pair	58	847	5^′	GTC CAC CGT GTA TGC CTT CT
				3^′	TCG ATG TCG ATG GTA CGA AA
	Inner pair	45	278	5^′	GGT TCG AGC CCA ATT TTA CA
				3^′	GGG CAC GCG TCC ATG TCG GC
prdx2 NM_011563	Outer pair	56	946	5^′	TAG GGC TCT CTC GGT TTT GA
				3^′	TTG ACT GTG ATC TGG CGA AG
	Inner pair	56	179	5^′	GGT GCC TTC AAG GAA ATC AA
				3^′	GCC TAG CTT TCG GAA GTC CT