Selection of the In Vitro Culture Media Influences mRNA Expression of Hedgehog Genes, Il-6, and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos

Table 2

Number of zygotes developed to blastocyst in correlation with gene expression of the mRNAs of interest.

	In vivo	In vitro in COOK cleavage medium	In vitro in Vitrolife’s G-1 PLUS medium
Number of zygotes	unknown	140	140
Number of good quality blastocysts	106	92	78
No. of blastocyst with detectable mRNA expression of
β-actin	106/106	92/92	78/78
shh	8/106	12/92	17/78
ihh	4/106	10/92	26/78
il-6	27/106	72/92	43/78
nox	0/106	2/92	1/78
gpx4	98/106	92/92	77/78
gpx1	42/106	79/92	72/78
prdx2	39/106	91/92	76/78