The Effects of α-Lipoic Acid against Testicular Ischemia-Reperfusion Injury in Rats

Figure 5

Effects of ischemia/reperfusion and LA (100 mg/kg ip, 30 minutes prior to detorsion) on germ cell apoptosis, TUNEL (a1–5), and active caspase-3 immunoreactivity (b1–5) in the testis tissue. TUNEL-positive and caspase-3-positive cells enhanced in the I (a3–b3) and I/R groups (a4–b4). LA pretreatment significantly reduced the number of apoptotic cells (a5–b5). Arrows indicate TUNEL-positive and caspase-3-positive cells.