Assessment of Control Tissue for Gene and Protein Expression Studies: A Comparison of Three Alternative Lung Sources

Figure 1

Quantitative real-time PCR analysis of VEGFA (a), PGF (b), FLT1 (c), KDR (d), MMP-2 (e), MMP-9 (f), TIMP-1 (g), and TIMP-2 (h). Gene expression levels were increased for VEGFA, PGF, FLT1, KDR, MMP-2, TIMP-1, and TIMP-2 in lung cancer (𝑛=29) compared to formalin-fixed paraffin-embedded (FFPE) tissue (𝑛=7) and stable lung transplant biopsies (𝑛=26). The levels of MMP-9 were significantly decreased. Results are expressed as mean ± SD. Asterisks indicate 𝑃<0.05.

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