Research Article

Distinct Mechanisms Regulating Gene Expression Coexist within the Fermentative Pathways in Chlamydomonas reinhardtii

Figure 2

Expression patterns for LDH1, ADH2, PDC3, PFL1, and PFR1 in synchronized Chlamydomonas cells over 24-hour cycles are shown in continuous dark (left, shaded box) and continuous light (right, white box), with samples taken every 4 hours. Cells were grown at 23°C. The bar on top of each graph depicts the corresponding virtual day/night periods. Relative expression levels were measured by real-time reverse-transcription polymerase chain reaction (qPCR) (1 = the value of expression measured at the first data point which corresponds to 8:00 on the first day). Data are mean ± SD, 𝑛 = 3 replicates. When not shown the error bars were smaller than the symbols.
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