Distinct Mechanisms Regulating Gene Expression Coexist within the Fermentative Pathways in Chlamydomonas reinhardtii
Figure 2
Expression patterns for LDH1, ADH2, PDC3, PFL1, and PFR1 in synchronized Chlamydomonas cells over 24-hour cycles are shown in continuous dark (left, shaded box) and continuous light (right, white box), with samples taken every 4 hours. Cells were grown at 23°C. The bar on top of each graph depicts the corresponding virtual day/night periods. Relative expression levels were measured by real-time reverse-transcription polymerase chain reaction (qPCR) (1 = the value of expression measured at the first data point which corresponds to 8:00 on the first day). Data are mean ± SD, replicates. When not shown the error bars were smaller than the symbols.