Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

Table 1

Timetable for pump configuration.

Time (min)	Flow rate (μL/min)
	Eluent A	Eluent B
0.00	300	400
0.05	4500	300
0.75	4500	400
0.80	4500	300
0.85	300	300
2.20	300	300
2.25	4500	300
2.45	3300	300
2.50	300	300

Eluent A: MeOH/H₂O (50/50, v/v); eluent B: MeOH/H₂O (97/3, v/v; 10 mmol/L CH₃COONH₄, 0.1% acetic acid).