Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

Table 2

LC-MS/MS parameters (MRM transition, DP, EP, CEP, CE, and CXP) and retention times of four immunosuppressive drugs and internal standards.

	CyA	TRL	SRL	RAD	Ascomycin IS	CyD IS
MRM-transition	1202.80 → 425.20	821.60 → 768.50	931.66 → 864.50	975.65 → 908.50	809.59 → 756.40	1216.90 → 425.50
DP (V)	100.00	50.63	41.25	44.38	50.63	110.00
EP (V)	10	10	10	10	10	10
CEP (V)	39.85	34.88	38.52	39.97	34.49	47.93
CE (V)	68	29	23	250	29	69
CXP (V)	4	12	14	14	12	4
Retention time (min)	1.97	1.81	1.84	1.85	1.81	2.00
Retention time RSD% (min)	0.245	0.137	0.186	0.142	0.148	0.236

CyA: cyclosporin A, TRL: tacrolimus, SRL: sirolimus, RAD: everolimus, CyD: cyclosporin D, IS: internal Standard, MRM: multiple reaction monitoring, DP: declustering potential, EP: entrance potential, CEP: collision cell entrance potential, CE: collision energy, CXP: collision cell exit potential, and V: volt.