Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

Table 3

Method performance parameters.

	CyA	TRL	SRL	RAD
LOD (μg/L)	5.6	1.4	0.72	1.15
LOQ (μg/L)	15.4	4	1.8	3.1
Linearity^a (𝑅^2)	0.9998	0.9998	0.9998	0.9998
Recovery^b (%)
Concentration 1	102.1	95	94.3	95.9
Concentration 2	98.5	92.8	95.7	94.9
Intraday CV (%) (LI)^c	3.2	2.8	3.2	2.4
Interday CV (%) (LI)^c	3.2	4.3	4.5	4.1
Intraday CV (%) (LII)^d	3.3	2.2	2.1	1.7
Interday CV (%) (LII)^d	4.7	4.3	4.3	5.9
Intraday CV (%) (III)^e	4.5	3.5	4.8	3.0
Interday CV (%) L(III)^e	6.2	3.8	3.0	3.99
Accuracy (%)	100.2	99.0	100.3	97.9

CyA: cyclosporin A, TRL: tacrolimus, SRL: sirolimus, RAD: everolimus, and LOD: limit of detection, LOQ: lover limits of quantification.
^a𝑐=15.4−4400 μg/L (CyA), 1.8–250 μg/L (SRL), 3.1–200 μg/L (RAD), and 4–200 μg/L (TRL).
^b𝑐1=100 μg/L (CyA), 𝑐1=10 μg/L (SRL, RAD, TRL), 𝑐2=500 μg/L (CyA), and 𝑐2=100 μg/L for (SRL, RAD, and TRL).
^c𝑐=50 μg/L (CyA), 𝑐=4 μg/L (TRL), 𝑐=1.8 μg/L (SRL), 𝑐=3.1 μg/L (RAD).
^d𝑐=100 μg/L (CyA), 𝑐=20 μg/L (TRL), 𝑐=7.2 μg/L (SRL), and 𝑐=12.4 μg/L (RAD).
^e𝑐=500 μg/L (CyA), 𝑐=40 μg/L (TRL), 𝑐=18 μg/L (SRL), and 𝑐=31 μg/L (RAD).