Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers

Table 2

Optimisation of DALP primers used for the PCR protocols.

Primers	Sequence	Mg+2 conc.	Taq polymerase used	Annealing temperature (°C)
DALP231F	5′-GTTTTCCCAGTCACGACAGC-3′	1.8 mM	Promega	55
DALP234F	5′-GTTTTCCCAGTCACGACCAG-3′	1.8 mM	Promega	55
DALP235F	5′-GTTTTCCCAGTCACGACCAC-3′	1.8 mM	Promega	55
Universal DALPR	5′-TTTCACACAGGAAACAGCTATGAC-3′