Research Article
Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers
Table 3
Frequency of presence of bands (%) markers in Nilaparvata spp. obtained from three DALP primers.
| Primer | Marker (M. Wt.) | UPM | Tanjung Karang | Melaka | Perak | Sabah | Cameron High lands | | N. lugens (Rice) | N. lugens (Leersia) | N. lugens (Rice) | N. lugens (Leersia) | N. lugens (Rice) | N.lugens (Leersia) | N. lugens (Rice) | N.lugens (Leersia) | N. lugens (Rice) | N.lugens (Leersia) | N.bakeri (Leersia) |
| | DALP231.1 (2.15 kb) | 10 | 10 | 0 | 0 | 10 | 20 | 10 | 0 | 45 | 10 | 0 | | DALP231.2 (2.0 kb) | 0 | 25 | 0 | 0 | 40 | 30 | 15 | 0 | 50 | 10 | 30 | | DALP231.3 (1.9 kb) | 25 | 0 | 0 | 0 | 40 | 35 | 15 | 10 | 25 | 10 | 0 | | DALP231.4 (1.8 kb) | 100 | 65 | 100 | 90 | 100 | 90 | 100 | 80 | 70 | 100 | 80 | | DALP231.5 (1.7 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 90 | | DALP231.6 (1.6 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP231.7 (1.4 kb) | 60 | 100 | 100 | 80 | 50 | 100 | 100 | 60 | 70 | 100 | 90 | | DALP231.8 (1.3 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP231.9 (1.2 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP231.10 (1.0 kb) | 0 | 60 | 0 | 50 | 0 | 40 | 0 | 60 | 0 | 40 | 100 | DALP231 | DALP231.11 (0.9 kb) | 0 | 10 | 10 | 0 | 0 | 20 | 0 | 0 | 0 | 30 | 0 | | DALP231.12 (0.85 kb) | 30 | 40 | 25 | 50 | 30 | 30 | 20 | 30 | 40 | 35 | 80 | | DALP231.13 (0.75 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP231.14 (0.70 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP231.15 (0.60 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 30 | 30 | 0 | | DALP231.16 (0.50 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP231.17 (0.40 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP231.18 (0.30 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP231.19 (0.25 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP231.20 (0.20 kb) | 80 | 60 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP231.21 (0.10 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP231.22 (0.09 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP234.1 (2.0 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP234.2 (1.9 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP234.3 (1.8 kb) | 20 | 50 | 25 | 50 | 20 | 50 | 20 | 30 | 30 | 50 | 0 | | DALP234.4 (1.75 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP234.5 (1.70 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP234.6 (1.65 kb) | 0 | 70 | 0 | 40 | 0 | 40 | 0 | 60 | 0 | 40 | 100 | DALP234 | DALP234.7 (1.6 kb) | 0 | 10 | 30 | 0 | 0 | 20 | 0 | 0 | 0 | 30 | 0 | | DALP234.8 (1.5 kb) | 40 | 40 | 60 | 50 | 30 | 30 | 20 | 30 | 40 | 35 | 100 | | DALP234.9 (1.45 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP234.10 (1.3 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP234.11 (1.25 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 30 | 30 | 0 | | DALP234.12 (1.10 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP234.13 (1.0 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP234.14 (0.9 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP234.15 (0.85 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP234.16 (0.7 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP234.17 (0.65 kb) | 90 | 90 | 100 | 100 | 100 | 100 | 90 | 100 | 100 | 90 | 100 | | DALP234.18 (0.5 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP234.19 (0.35 kb) | 40 | 50 | 100 | 100 | 100 | 100 | 100 | 100 | 80 | 90 | 100 | | DALP234.20 (0.30 kb) | 80 | 20 | 80 | 70 | 60 | 40 | 50 | 90 | 70 | 80 | 100 | | DALP234.21 (0.15 kb) | 40 | 50 | 90 | 100 | 80 | 100 | 100 | 100 | 80 | 90 | 100 | | DALP235.1 (2.2 kb) | 5 | 10 | 10 | 8 | 0 | 10 | 9 | 20 | 40 | 35 | 0 | | DALP235.2 (2.1 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP235.3 (2.0 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP235.4 (1.9 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP235.5 (1.8 kb) | 10 | 40 | 15 | 50 | 20 | 35 | 16 | 30 | 10 | 50 | 0 | | DALP235.6 (1.75 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP235.7 (1.70 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP235.8 (1.65 kb) | 0 | 60 | 0 | 50 | 0 | 40 | 0 | 60 | 0 | 40 | 100 | | DALP235.9 (1.6 kb) | 0 | 10 | 10 | 0 | 0 | 20 | 0 | 0 | 0 | 30 | 0 | | DALP235.10 (1.5 kb) | 30 | 40 | 25 | 50 | 30 | 30 | 20 | 30 | 40 | 35 | 100 | | DALP235.11 (1.4 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP235.12 (1.3 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP235.13 (1.2 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 30 | 30 | 0 | | DALP235.14 (1.15 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP235.15 (1.0 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | DALP235 | DALP235.16 (0.9 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP235.17 (0.8 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP235.18 (0.7 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 0 | | DALP235.19 (0.6 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | | DALP235.20 (0.5 kb) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | | DALP235.21 (0.35 kb) | 30 | 50 | 100 | 100 | 100 | 100 | 100 | 100 | 80 | 90 | 70 | | DALP235.22 (0.30 kb) | 90 | 20 | 80 | 70 | 60 | 40 | 50 | 90 | 70 | 80 | 100 | | DALP235.23 (0.20 kb) | 60 | 90 | 50 | 30 | 80 | 100 | 90 | 60 | 40 | 75 | 100 | | DALP235.24 (0.15 kb) | 100 | 100 | 80 | 100 | 100 | 100 | 100 | 100 | 90 | 100 | 90 | | DALP235.25 (0.10 kb) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
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Total Number of markers | 45 | 51 | 46 | 46 | 45 | 52 | 46 | 47 | 49 | 55 | 41 |
| Number of polymorphic markers | 17 | 23 | 14 | 14 | 13 | 18 | 13 | 15 | 21 | 25 | 7 |
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% Polymorphic markers | 37.77 | 45.09 | 30.43 | 30.43 | 28.88 | 34.61 | 28.26 | 31.91 | 42.85 | 45.45 | 17.08 |
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Overall number of markers based on the populations of N. lugens | | 68 |
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Overall number of polymorphic markers based on the populations of N. lugens | | 29 |
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Overall % poymorphism based on the populations of N. lugens | | 42.64 |
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Frequency based upon 20 individuals per population.
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