Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential
Figure 6
Expression of differentiation markers of the keratinocytes cultured on XD at the air-liquid interface (A2, B2, C2, D2) compared to the normal skin (A1, B1, C1, D1), to freshly healing wound after necrectomy treated with XD (A3, B3, C3, D3), and to freshly healing wound after necrectomy treated with Grassolind (A4, B4, C4, D4). The wounds after necrectomy were covered with XD (column 3) or tulle gras Grassolind (column 4). On day 8 after treatment, biopsies were taken. The expression of differentiation markers HMW CK (a), involucrin (b), p63 (c), and CD29 (d) was compared in the normal skin (column 1), in keratinocytes cultured on XD (column 2), in the wound treated with XD (column 3), and in the wound treated with Grassolind as a control (column 4). The figures show that keratinocyte growth, and differentiation in vitro on XD (column 2) and in vivo epithelization under XD (column 3) is similar and that the wound covered with XD formed epidermis within one week (column 3), while the wound covered with Grassolind did not epithelize (column 4), although remnants of keratinocytes (A4) or adnexa remnants (C4) are visible in the dermis (arrows). Note that cells migrated into the XD matrix formed islands resembling primitive gland-like structures (C2, D2—arrows); they are p63− in the centre and p63+ at the outer margin of the colony (compare to gland remnants in C3 and D3—arrows). Bars—50 μm.
