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The Scientific World Journal
Volume 2012 (2012), Article ID 821062, 14 pages
http://dx.doi.org/10.1100/2012/821062
Research Article

New Insights in the Sugarcane Transcriptome Responding to Drought Stress as Revealed by Supersage

1Department of Genetics, Federal University of Pernambuco (UFPE), 50670-901 Recife, PE, Brazil
2Biotechnology Division, Sugarcane Technology Center (CTC), 13400-970 Piracicaba, SP, Brazil

Received 31 October 2011; Accepted 2 December 2011

Academic Editor: Luigi Cattivelli

Copyright © 2012 Éderson Akio Kido et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the scope of the present work, four SuperSAGE libraries have been generated, using bulked root tissues from four drought-tolerant accessions as compared with four bulked sensitive genotypes, aiming to generate a panel of differentially expressed stress-responsive genes. Both groups were submitted to 24 hours of water deficit stress. The SuperSAGE libraries produced 8,787,315 tags (26 bp) that, after exclusion of singlets, allowed the identification of 205,975 unitags. Most relevant BlastN matches comprised 567,420 tags, regarding 75,404 unitags with 164,860 different ESTs. To optimize the annotation efficiency, the Gene Ontology (GO) categorization was carried out for 186,191 ESTs (BlastN against Uniprot-SwissProt), permitting the categorization of 118,208 ESTs (63.5%). In an attempt to elect a group of the best tags to be validated by RTqPCR, the GO categorization of the tag-related ESTs allowed the in silico identification of 213 upregulated unitags responding basically to abiotic stresses, from which 145 presented no hits after BlastN analysis, probably concerning new genes still uncovered in previous studies. The present report analyzes the sugarcane transcriptome under drought stress, using a combination of high-throughput transcriptome profiling by SuperSAGE with the Solexa sequencing technology, allowing the identification of potential target genes during the stress response.