Characterization of Mononucleated Human Peripheral Blood Cells

Figure 2

Expression of hematopoietic stem cell (HSC) and mesenchymal stem cell (MSC) markers in mononucleated cells derived from peripheral blood. RT-PCR analysis was performed using total RNA isolated from adherent and suspension mononucleated cells of human peripheral blood. CD105 was used as an MSC marker, SLAM F1 as an HSC marker, and KIT as a stem cell factor (SCF) marker. GAPDH was used as a positive control for RT-PCR analysis. A 100 bp DNA ladder was used to identify the approximate size of the RT-PCR products. The RT-PCR products were observed by electrophoresis on 1% (w/v) agarose gels and stained with ethidium bromide. All RT-PCR products were confirmed with DNA sequencing after cloning and were found to be 100% identical to the known sequences obtained from BLAST analysis.