Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)

Table 1

Effect of different concentrations of ethylene inhibitors on on shoot regeneration and growth from leaf cultures of Sinningia speciosa after 6 weeks in culture on regeneration medium (MS medium with 2.0 mg/L BA and 0.1 mg/L NAA).

Ethylene inhibitors*; (mg L^−1)		Regeneration frequency**; (%)	Number of shoots per explant**	Shoot length^a; (cm)
Control	0	72	12.3 ± 0.8	1.21 ± 0.1
AVG	1	85	14.3 ± 1.2	1.23 ± 0.2
	5	75	13.5 ± 0.9	1.6 ± 0.2
	10	64	10.6 ± 0.7	1.8 ± 0.2
	20	58	8.2 ± 0.4	1.1 ± 0.1
CoCl2	1	79	13.8 ± 0.7	1.36 ± 0.1
	5	72	11.8 ± 0.9	1.18 ± 0.1
	10	62	9.2 ± 0.6	0.85 ± 0.1
	20	45	5.9 ± 0.4	0.57 ± 0.1
STS	1	77	13.1 ± 0.9	1.18 ± 0.1
	5	91	17.2 ± 1.6	1.27 ± 0.2
	10	89	16.8 ± 1.2	1.25 ± 0.2
	20	63	11.4 ± 0.8	0.84 ± 0.1

^*Basal medium consisted of MS salts and vitamins, 30 g L⁻¹ sucrose, and 2 mg/L BAP, solidified with 7 g/L Phytagar.
^**From 100 leaf explants tested.
^aValues represent the mean ± standard deviation of 50 shoots.