Research Article

Distribution of BoLA-DRB3 Allelic Frequencies and Identification of Two New Alleles in Iranian Buffalo Breed

Table 1

Frequencies of BoLA-DRB3.2 alleles in Iranian Mazandarani Buffalo () as identified by polymerase chain reaction and restriction fragment length polymorphism analysis. Corresponding DNA patterns are shown at the bottom of the figures. Nomenclature of DNA patters is given corresponding to http://www.projects.roslin.ac.uk/bola/drb3pcr.html. Standard errors of the allele frequencies do not exceed 5%.

AllelesDRB3 PCR-RFLP RsaI/BstYI/
HaeIII patterns
Frequencies
(%)

DRB3 *3901 *48Wba24.20
DRB3 *2301, *2901, *3601 *20Ibb14.52
DRB3 *0801 *21Ibe14.00
New newObe10.75
DRB3 *2601 *19Sbb10.22
DRB3 *0401 *13Hba9.14
DRB3 *2802 *42Hbf8.51
DRB3 *3701 *49Wbe2.70
DRB 07 *37Oba2.70
DRB3 *25012 *43Kbf1.08
DRB3 *4001 *50Xba1.08
Newnewgbb1.08