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The Scientific World Journal
Volume 2012 (2012), Article ID 906873, 5 pages
Comparison of Two Methodologies for CD4+ T Lymphocytes Relative Counting on Immune Monitoring of Patients with Human Immunodeficiency Virus
1Pharmaceutical Sciences Post Graduate Program, State University of Ponta Grossa (UEPG), General Carlos Cavalcanti Avenue, 4748 Uvaranas, 84030-900 Ponta Grossa, PR, Brazil
2Department of Pharmacy, State University in the Paraná Midwestern (UNICENTRO), CEDETEG Campus, 85040-080 Guarapuava, PR, Brazil
3County Health Department, Expert Assistance Service (EAS), City Hall, 84051-900 Ponta Grossa, PR, Brazil
4Department of Physiotherapy, State University Paulista (UNESP), Presidente Prudente Campus, 19060-900 Presidente Prudente, SP, Brazil
Received 1 September 2012; Accepted 8 November 2012
Academic Editors: A. Banerjee and E. L. Hooghe-Peters
Copyright © 2012 Danielle Cristyane Kalva Borato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- T. H. Mogensen, J. Melchjorsen, C. S. Larsen, and S. R. Paludan, “Innate immune recognition and activation during HIV infection,” Retrovirology, vol. 7, article 54, 2010.
- B. Moll, E. E. Emeson, and C. B. Small, “Inverted ratio of inducer to suppressor T lymphocyte subsets in drug abusers with opportunistic infections,” Clinical Immunology and Immunopathology, vol. 25, no. 3, pp. 417–423, 1982.
- J. B. Alimonti, T. B. Ball, and K. R. Fowke, “Mechanisms of CD4+ T lymphocyte cell death in human immunodeficiency virus infection and AIDS,” Journal of General Virology, vol. 84, no. 7, pp. 1649–1661, 2003.
- X. Li, C. Breukers, A. Ymeti, B. Lunter, L. W. M. M. Terstappen, and J. Greve, “CD4 and CD8 enumeration for HIV monitoring in resource-constrained settings,” Cytometry B, vol. 76, no. 2, pp. 118–126, 2009.
- Centers for Disease Control and Prevention, “1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV),” Morbidity and Mortality Weekly Report, vol. 46, no. RR-2, pp. 1–29, 1997.
- M. R. G. O'Gorman and J. K. A. Nicholson, “Adoption of single-platform technologies for enumeration of absolute T lymphocyte subsets in peripheral blood,” Clinical and Diagnostic Laboratory Immunology, vol. 7, no. 3, pp. 333–335, 2000.
- C. A. Janeway, T. Paul, and M. Walport, Immunobiology, Garland Publishing, New York, NY, USA, 6th edition, 2005.
- G. Janossy, I. V. Jani, N. J. Bradley, A. Bikoue, T. Pitfield, and D. K. Glencross, “Affordable CD4+-T-cell counting by flow cytometry: CD45 gating for volumetric analysis,” Clinical and Diagnostic Laboratory Immunology, vol. 9, no. 5, pp. 1085–1094, 2002.
- J. Hoffman, J. Van Griensven, R. Colebunders, and M. McKellar, “Role of the CD4 count in HIV management,” HIV Therapy, vol. 4, no. 1, pp. 27–39, 2010.
- H. Bussmann, C. W. Wester, K. V. Masupu et al., “Low CD4+ T lymphocyte values in human immunodeficiency virus-negative adults in Botswana,” Clinical and Diagnostic Laboratory Immunology, vol. 11, no. 5, pp. 930–935, 2004.
- J. P. Aboulker, B. Autran, K. Beldjord, F. Touraine, and P. Debre, “Consistency of routine measurements of CD4+, CD8+ peripheral blood lymphocytes,” Journal of Immunological Methods, vol. 154, no. 2, pp. 155–161, 1992.
- D. M. Moore, R. S. Hogg, B. Yip, K. Craib, E. Wood, and J. S. G. Montaner, “CD4 percentage is an independent predictor of survival in patients starting antiretroviral therapy with absolute CD4 cell counts between 200 and 350 cells/microL,” HIV Medicine, vol. 7, no. 6, pp. 383–388, 2006.
- Y. Pirzada, S. Khuder, and H. Donabedian, “Predicting AIDS-related events using CD4 percentage or CD4 absolute counts,” AIDS Research and Therapy, vol. 3, no. 1, article 20, 2006.
- M. Guiguet, E. Kendjo, G. Carcelain et al., “CD4+ T-cell percentage is an independent predictor of clinical progression in AIDS-free antiretroviral-naive patients with CD4+ T-cell counts >200 cells/mm3,” Antiviral Therapy, vol. 14, no. 3, pp. 451–457, 2009.
- T. Hulgan, S. Raffanti, A. Kheshti et al., “CD4 lymphocyte percentage predicts disease progression in HIV-infected patients initiating highly active antiretroviral therapy with CD4 lymphocyte counts >350 Lymphocytes/mm3,” Journal of Infectious Diseases, vol. 192, no. 6, pp. 950–957, 2005.
- T. Hulgan, B. E. Shepherd, S. P. Raffanti et al., “Absolute count and percentage of CD4+ lymphocytes are independent predictors of disease progression in HIV-infected persons initiating highly active antiretroviral therapy,” Journal of Infectious Diseases, vol. 195, no. 3, pp. 425–431, 2007.
- E. Simson and W. Groner, “Variability in absolute lymphocyte counts obtained by automated cell counters,” Communications in Clinical Cytometry, vol. 22, no. 1, pp. 26–34, 1995.
- B. Brando, D. Barnett, G. Janossy, et al., “Cytofluorometric methods for assessing absolute numbers of cell subsets in blood,” Cytometry, vol. 42, no. 6, pp. 327–346, 2000.
- D. Glencross, L. E. Scott, I. V. Jani, D. Barnett, and G. Janossy, “CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration,” Clinical Cytometry, vol. 50, no. 2, pp. 69–77, 2002.
- C. A. MacLennan, M. K. P. Liu, S. A. White et al., “Diagnostic accuracy and clinical utility of a simplified low cost method of counting CD4 cells with flow cytometry in Malawi: diagnostic accuracy study,” British Medical Journal, vol. 335, no. 7612, pp. 190–194, 2007.