An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

Figure 2

The sensitivity and specificity achieved with primer pair SZNP-F2/R2 in SYBR Green real-time PCR. (a) Standard curve. LogCO: log10 (copies), slope: −3.4779, 𝑅²: 0.998, eff%: 110%. (b) Melting curve. (c) Agarose electrophoresis of PCR products of H5N1. Lane 1: DL2000 Marker; Lanes 2–9: 10⁸–10¹ copies of H5N1 virus cDNA.

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