Figure 2: The sensitivity and specificity achieved with primer pair SZNP-F2/R2 in SYBR Green real-time PCR. (a) Standard curve. LogCO: log10 (copies), slope: −3.4779, : 0.998, eff%: 110%. (b) Melting curve. (c) Agarose electrophoresis of PCR products of H5N1. Lane 1: DL2000 Marker; Lanes 2–9: 108–101 copies of H5N1 virus cDNA.