An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

Table 3

Ct values of ferret or monkey cDNAs prepared by five different methods and subjected to SYBR-Green qPCR.

	RO	AM	BI	QI	TR
Ferret lung	20.51	18.54	18.85	17.99	17.31
Ferret small intestine	18.29	18.36	18.31	18.29	18.25
Monkey lung	18.31	18.39	18.58	18.34	18.43
Monkey small intestine	20.11	20.26	19.32	17.05	20.18

Note: The Ct values shown are the average of at least 3 replicates with standard deviations of 6.0–10%. The P values from t-test calculations were >0.05 between the different methods.