Research Article
Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe
Table 1
Effect of the addition order of reagents on the enhanced fluorescence intensity (ΔF%).
| Reagent | Reagent | Reagent | Reagent | ΔF% |
| Tb3+ | Dano | Albumin | Buffer | 46.6 | Tb3+ | Dano | Buffer | Albumin | 37.5 | Tb3+ | Buffer | Dano | Albumin | 30.9 | Tb3+ | Buffer | Albumin | Dano | 36.9 | Tb3+ | Albumin | Dano | Buffer | 34.7 | Tb3+ | Albumin | Buffer | Dano | 43.5 | Dano | Tb3+ | Albumin | Buffer | 39.5 | Dano | Tb3+ | Buffer | Albumin | 41.7 | Dano | Buffer | Tb3+ | Albumin | 37.3 | Dano | Buffer | Albumin | Tb3+ | 44.2 | Dano | Albumin | Buffer | Tb3+ | 43.0 | Dano | Albumin | Tb3+ | Buffer | 41.3 | Buffer | Tb3+ | Dano | Albumin | 26.5 | Buffer | Tb3+ | Albumin | Dano | 24.5 | Buffer | Albumin | Dano | Tb3+ | 40.7 | Buffer | Albumin | Tb3+ | Dano | 33.6 | Buffer | Dano | Albumin | Tb3+ | 41.7 | Buffer | Dano | Tb3+ | Albumin | 40.1 | Albumin | Tb3+ | Dano | Buffer | 37.6 | Albumin | Tb3+ | Buffer | Dano | 40.1 | Albumin | Dano | Buffer | Tb3+ | 39.5 | Albumin | Dano | Tb3+ | Buffer | 43.1 | Albumin | Buffer | Tb3+ | Dano | 31.9 | Albumin | Buffer | Dano | Tb3+ | 25.8 |
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Analytical conditions: [Tb3+]: 8.5 × 10−5 mol L−1; [Dano]: 1.5 × 10−4 mol L−1; [HSA]: 1 × 10−6 mol L−1; pH = 7.8.
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