Spectrofluorimetric Determination of Human Serum Albumin Using Terbium-Danofloxacin Probe

Table 1

Effect of the addition order of reagents on the enhanced fluorescence intensity (ΔF%).

Reagent	Reagent	Reagent	Reagent	ΔF%
Tb^3+	Dano	Albumin	Buffer	46.6
Tb^3+	Dano	Buffer	Albumin	37.5
Tb^3+	Buffer	Dano	Albumin	30.9
Tb^3+	Buffer	Albumin	Dano	36.9
Tb^3+	Albumin	Dano	Buffer	34.7
Tb^3+	Albumin	Buffer	Dano	43.5
Dano	Tb^3+	Albumin	Buffer	39.5
Dano	Tb^3+	Buffer	Albumin	41.7
Dano	Buffer	Tb^3+	Albumin	37.3
Dano	Buffer	Albumin	Tb^3+	44.2
Dano	Albumin	Buffer	Tb^3+	43.0
Dano	Albumin	Tb^3+	Buffer	41.3
Buffer	Tb^3+	Dano	Albumin	26.5
Buffer	Tb^3+	Albumin	Dano	24.5
Buffer	Albumin	Dano	Tb^3+	40.7
Buffer	Albumin	Tb^3+	Dano	33.6
Buffer	Dano	Albumin	Tb^3+	41.7
Buffer	Dano	Tb^3+	Albumin	40.1
Albumin	Tb^3+	Dano	Buffer	37.6
Albumin	Tb^3+	Buffer	Dano	40.1
Albumin	Dano	Buffer	Tb^3+	39.5
Albumin	Dano	Tb^3+	Buffer	43.1
Albumin	Buffer	Tb^3+	Dano	31.9
Albumin	Buffer	Dano	Tb^3+	25.8

Analytical conditions: [Tb³⁺]: 8.5 × 10⁻⁵ mol L⁻¹; [Dano]: 1.5 × 10⁻⁴ mol L⁻¹; [HSA]: 1 × 10⁻⁶ mol L⁻¹; pH = 7.8.