B Cell Responses to CpG Correlate with CXCL16 Expression Levels in Common Variable Immunodeficiency

Figure 6

CXCL16^high B cell responses to CpG. (a) Evaluation of target gene (mRNA levels) upregulation (mRNA levels) after in vitro stimulation with CpG. Fold increase of CD69, CD86, TLR9, AICDA, and IL-6 at the mRNA level after CpG stimulation. Experiments were performed with PBMCs in triplicates for all patients (𝑛=15) and healthy controls (𝑛=8). (b) Fold increase of TLR9, AICDA, and IL-6 at the mRNA level after CpG stimulation. Experiments were performed with purified B cells from patients (𝑛=5) and healthy controls (𝑛=2). Graph bars show standard error (SE). (c) Evaluation of target gene (mRNA levels) upregulation (mRNA levels) after TNF-α blockade and in vitro stimulation with CpG. Fold variation of CD69, CD86, TLR9, AICDA, and IL-6 at the mRNA level is showed. Experiments were performed with PBMCs in triplicates for all patients (𝑛=15) and healthy controls (𝑛=8). Graph bars show standard error (SE).

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