Characterization of eDNA from the Clinical Strain Acinetobacter baumannii AIIMS 7 and Its Role in Biofilm Formation
Figure 4
PCR amplification of 16SrRNA, 5โฒcoding region of bap and from genomic DNA and eDNA. Agarose gel (1.5%) showing (a): Lane M: Molecular weight marker (values in kb), Lane 1: 16SrRNA (1.5โkb) amplified from genomic DNA, and Lane 2: 16SrRNA (1.5โkb) amplified from eDNA. Lane 3: DNase, RNase, Protease-free water as negative PCR control. (b): Lane M: Molecular weight marker (values in kb), Lane 1: 1019โbp sized 5โฒcoding region of bap gene amplified from genomic DNA, and Lane 2: 1019โbp sized 5โฒcoding region of bap gene amplified from eDNA, Lane 3: DNase, RNase, Protease-free water as negative PCR control. (c): Lane M: Molecular weight marker (values in kb), Lane 1: 875โbp gene amplified from genomic DNA, Lane 2: 875โbp gene amplified from eDNA, and Lane 3: DNase, RNase, Protease-free water as negative PCR control.
