The Effects of Piroxicam and Deracoxib on Canine Mammary Tumour Cell Line
Figure 4
Flow cytometric analysis of apoptosis of coadministration of piroxicam and deracoxib on canine mammary carcinoma cell line after 72 h incubation. The lower left quadrant of the histogram shows the viable cells, which exclude PI, and are negative for FITC-Annexin-V-binding FITC−/PI− (LL). The lower right compartment represents the early apoptotic cells, which are PI negative and Annexin-V positive, indicating the translocation of phosphatidylserine to the external cell surface FITC+/PI− (LR). The upper right quadrant represents the late-stage apoptotic cells which are PI and Annexin-V positive FITC+/PI+ (UR), and the upper left compartment shows the necrotic cells which are only PI positive FITC−/PI+ (UL). The numbers written on histograms represent the sum of early and late apoptotic cells (%).
