Influence of Codon Bias on Heterologous Production of Human Papillomavirus Type 16 Major Structural Protein L1 in Yeast

Figure 1

Analysis of HPV-16 L1 mRNA expression in transformed yeast cells by Northern blot. Total yeast RNA was isolated 4 h after induction from yeast transformants expressing five different HPV-16 L1 ORFs and control yeast cells transformed with the empty pFX7 vector. Fifteen micrograms of total RNA was loaded per lane and hybridized with L1-Pv, L1-Ec, L1-Pl, L1-Hm, and PGK1-control cDNA probes labeled with [α-33P]-dATP by random priming. The data from one representative experiment are shown. The 4 independent experiments with other randomly picked transformants in every group were performed with similar results.