Supercooling Agent Icilin Blocks a Warmth-Sensing Ion Channel TRPV3

Figure 3

(a) A representative recording from patch-clamp experiment showing camphor-induced mTRPV3 activity inhibited by 100 nM icilin (61% inhibition; 𝑛=6). HEK293 cells expressing mTRPV3 were held at –40 mV holding potential at ~33°C. (b) A representative recording from patch-clamp experiment showing 2-APB-induced mTRPV3 activity inhibited by 100 nM icilin (68% inhibition; 𝑛=7) under similar conditions as above. (c) A representative recording from patch-clamp experiment showing icilin-dependent (100 nM) inhibition of mTRPV3 activity induced by both camphor and 2-APB (74% inhibition; 𝑛=7) under similar conditions as above.

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