Evidence for Directed Evolution of Larger Size Motif in Arabidopsis thaliana Genome
Table 1
Frequency of occurrence of the various promoter sequences in which spacer sequence length between two ACGT palindromes is gradually increased from 5 to 25 nucleotides.
Cis element
Chromosome 1
Chromosome 2
Chromosome 3
Chromosome 4
Chromosome 5
Total
(ACGT) 2
ACGTACGT
469
312
367
327
410
1885
(ACGT) 8
ACGTACGTACGTACGTACGTACGTACGTACGT
70
31
12
28
59
200
(ACGT)N5(ACGT)
ACGTGGCTAACGT
16
11
13
13
19
72
(ACGT)N10(ACGT)
ACGTGGCTATGGCGACGT
8
5
10
4
12
39
(ACGT)N25(ACGT)
ACGTGGCTATGGCGGAGCAAGATTCACTCACGT
15
12
13
9
13
62
(ACGT)RN5(ACGT)
ACGT–GCTAG–ACGT
7
5
5
2
4
23
(ACGT)RN10(ACGT)
ACGT–TGGGGCCGAT–ACGT
2
2
4
3
3
14
(ACGT)RN25(ACGT)
ACGTAGACACGTTGGGGGAACTTACTGCCACGT
3
1
7
5
5
21
(ACGT)RN25(ACGT)
ACGT-ATATGAGATCGGCGCTTCACGGAGC-ACGT
4
14
6
4
4
32
(ACGT)N5(ACGT) randomized
GGAATCCTTGGCA
41
24
30
19
23
137
(ACGT)N10(ACGT) randomized
GCGGGCTATCGGTAGCAT
2
5
2
0
1
10
(ACGT)N25(ACGT) randomized
TAAGGCTTAGCCACGCTTAGGGTGTGAGCACAC
6
6
3
0
3
18
(TGCA)N25(TGCA)
TGCAGGCTATGGCGGAGCAAGATTCACTCTGCA
13
12
9
12
9
55
N5, N10, N25 denote sequence length between two ACGT palindromes. RN5, RN10, RN25—signify only spacer sequence being randomized. (ACGT) N_(ACGT) randomized—signify complete sequence being randomized.
