Research Article
A Real-Time PCR Assay for Bat SARS-Like Coronavirus Detection and Its Application to Italian Greater Horseshoe Bat Faecal Sample Surveys
Figure 2
Multiple sequence alignment of primer binding sites. The alignments include 10 SARS-related coronavirus reference strains: SBRp3 (Bat SARS CoV Rp3/2004, DQ071615, identified from Rhinolophus pearsoni), SBRs (SARS coronavirus Rs_672/2006, FJ588686, identified from Rhinolophus sinicus), SHTor2 (SARS coronavirus Tor2, AY274119, identified from Human), SC (Civet SARS CoV SZ16/2003, AY304488, identified from Paguma larvata), SB (SARS coronavirus isolate CFB/SZ/94/03, AY545919, identified from Melogale moschata), SBRf1 (Bat SARS CoV Rf1/2004, DQ412042, identified from Rhinolophus ferrumequinum), SBRm1 (Bat SARS CoV Rm1/2004, DQ412043, identified from Rhinolophus macrotis), SBHKU3-1 (Bat coronavirus HKU3, DQ022305, identified from Rhinolophus sinicus), SB273 (Bat CoV 273/2005, DQ648856, identified from Rhinolophus ferrumequinum), and SB279 (Bat CoV 279/2005, DQ648857, identified from Rhinolophus macrotis).
