Tissue Engineering of Urinary Bladder and Urethra: Advances from Bench to Patients
Figure 4
Mechanical stimuli-induced urothelial differentiation in a human tissue-engineered tubular genitourinary graft. Data from immunofluorescence were raised against the indicated molecule as presented in Cattan et al. [48]; they were analyzed with NIH ImageJ software. In (a) percentage of uroplakin II positive surface relative to the urothelial surface is depicted without stimulation (static) at day 7 (7 d), or day 14 (14 d), or with mechanical stimulation (dynamic) and compared with a native porcine tissue. In (b) similar data are presented for cytokeratin 20 (CK20). In (c) heparan sulphate (Heparan S) positive surface was evaluated compared to stromal surface. In (d) data depict collagen VII (Coll VII) positive pixels relative to the length in pixel of the basal lamina.
