The Scientific World Journal

Research Article

16S rRNA and Omp31 Gene Based Molecular Characterization of Field Strains of B. melitensis from Aborted Foetus of Goats in India

Table 1

Species and biovar differentiation of the species of the genus Brucella isolates.
Brucella isolatesSourceGrowth characteristicsMonospecific seraPhage typingInterpretation
UreaH2SCO2BFTHAMTbWbBK2FiIzR/C
P1Fetal membrane++−−++−+NLNLCLNLPLNLBrucella melitensis biovar 3
P2Stomach content++−−++−+NLNLCLNLPLNLBrucella melitensis biovar 3
P3Stomach content++−−++++NLNLCLNLPLNLBrucella melitensis biovar 3
P4Stomach content++−−++++NLNLCLNLPLNLBrucella melitensis biovar 3
P5Vaginal Swab++−−++++NLNLCLNLPLNLBrucella melitensis biovar 3
BF: basic fuchsin at 20  L/mL (1/50,000 w/v), TH: thionin at 20  L/mL (1/50,000 w/v), CL: confluent lysis, PL: partial lysis, NL: no lysis.