Association of FAS and FAS Ligand Genes Polymorphism and Risk of Systemic Lupus Erythematosus

Table 1

The primer sequences, annealing temperature, restriction enzymes, and fragments size of FAS and FASL polymorphisms.

Target sequence	Primer sequence	Annealing temperature	Restriction enzyme	PCR product	RFLP fragments
FAS A-670G; rs1800682	Forward: 5′-CTACCTAAGAGCTATCTACCGTTC-3′; Reverse: 5′-GGCTGTCCATGTTGTGGCTGC-3′[29]	61°C	MvaI (BstNI)	223	A: 223; G: 44, 189
FASL C-844T; rs763110	Forward: 5′-CAGCTACTCGGAGGCCAAG-3′; Reverse: 5′-GCTCTGAGGGGAGAGACCAT-3′[19]	63°C	BseMI (BsrDI)	401	T: 401; C: 223, 168