Intranuclear Crosstalk between Extracellular Regulated Kinase1/2 and Signal Transducer and Activator of Transcription 3 Regulates JEG-3 Choriocarcinoma Cell Invasion and Proliferation
Figure 4
STAT3 DNA-binding capacity in JEG-3 cells after ERK1/2 inhibition and stimulation with LIF. STAT3-DNA-binding capacities were assessed by an ELISA-based colorimetric assay. (a) Starved JEG-3 cells were treated or not with U0126 (10 nM) for 2 h and then stimulated or not with LIF (10 ng/mL) for another 30 min . (b) ERK1/2 expression was silenced by siRNA (silencing efficiency displayed by a representative Western blot, left upper corner) . Bars represent the mean of independent assays. Error bars show standard error of the mean. (Student’s -test).
