Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don

Figure 1

Morphogenesis of Lysionotus serratus. (a) Wild plant used for tissue culture, bar=2 cm. (b) Calli appeared at the cut edge of a leaf explant on medium containing 0.5 mg L⁻¹ KIN, bar=2 mm. (c) Adventitious shoot induced from leaf explant culture on the induction medium containing 0.5 mg L⁻¹ BA after 25 days, bar=2 mm. (d) High magnification of induced adventitious shoots. bar=1 mm. (e) Plantlet with well-established root system obtained by culturing leaf explant derived shoots on medium containing 0.5 mg L⁻¹ IBA for 45 days, bar=2 cm. (f) Regenerated plants 5 months after transplanting grew vigorously in a shaded greenhouse, bar=2 cm.

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