Shoot Organogenesis and Plant Regeneration from Leaf Explants of Lysionotus serratus D. Don

Table 2

Effects of PGRs on adventitious shoot proliferation in L. serratus.

PGR (mg L^−1)	Adventitious bud number per explant	Morphology of the explants; after 4 weeks of culture
0.1 BA	34.2±1.8c^z	Adventitious shoots
0.5 BA	52.6±2.4b	Adventitious shoots with 2.5% shoots vitrified
1.0 BA	77.2±4.8a	Adventitious shoots with 51.4% shoots vitrified
0.1 BA + 0.1 NAA	38.1±2.2c	Adventitious shoots with compact callus
0.5 BA + 0.1 NAA	49.2±2.5b	Adventitious shoots with compact callus, 5.5% shoots vitrified
1.0 BA + 0.1 NAA	66.4±4.3ab	Adventitious shoots, with compact callus, 34.2% shoots vitrified
0.1 TDZ	22.1±1.4d	Adventitious shoots, with few callus, shoot extremely small
0.5 TDZ	29.4±1.8cd	Adventitious shoots, with few green compact callus
1.0 TDZ	18.4±1.1d	Adventitious shoots, mass green compact callus, shoot extremely small
0.1 TDZ + 0.1 NAA	23.9±2.1d	Adventitious shoots, mass green compact callus
0.5 TDZ + 0.1 NAA	25.4±1.3d	Adventitious shoots, mass green compact callus, 8.5% shoots vitrified
1.0 TDZ + 0.1 NAA	33.5±1.5c	Adventitious shoots, mass green compact callus, 19.6% shoots vitrified

zMeans with the different letters within a column indicate significant differences based on Duncan’s new multiple range test (𝑃=0.05).