Evaluation of Genotoxic Effects of New Molecules with Possible Trypanocidal Activity for Chagas Disease Treatment
Table 3
Megazol induction of mutagenicity and cytotoxicity in Salmonella enterica serovar Typhimurium standard strains, without (−S9) and with (+S9) metabolic activation.
Strains
Dose (g/mL)
−S9
+S9
Mean ± S.D. (M.I.a)
Survivalb
Mean ± S.D. (M.I.a)
Survivalb
TA97
0
49 ± 19 (1.00)
100
123 ± 26 (1.00)
100
0.05
90 ± 10 (1.80)
100
176 ± 18 (1.40)
100
0.1
116 ± 7 (2.30*)
100
168 ± 7 (1.30)
100
0.2
142 ± 20 (2.80*)
100
273 ± 11 (2.20*)
100
0.5
165 ± 25 (3.30*)
100
408 ± 7 (3.30*)
80
1.0
266 ± 31 (5.40*)
100
548 ± 111 (4.40*)
80
TA98
0
27 ± 4 (1.00)
100
40 ± 4 (1.00)
100
0.05
38 ± 11 (1.30)
92
33 ± 1 (0.80)
100
0.1
49 ± 5 (1.80)
97
39 ± 7 (0.90)
100
0.2
81 ± 2 (2.90*)
100
41 ± 14 (1.00)
70
0.5
211 ± 22 (7.70*)
93
87 ± 2 (2.10*)
75
1.0
356 ± 35 (13.0*)
57
116 ± 14 (2.80*)
77
TA100
0
129 ± 12 (1.00)
100
171 ± 1 (1.00)
100
0.05
228 ± 13 (1.70)
72
324 ± 47 (1.80)
100
0.1
366 ± 59 (2.80*)
98
569 ± 58 (3.30*)
100
0.2
739 ± 29 (5.70*)
70
691 ± 106 (4.00*)
100
0.5
1302 ± 247 (10.0*)
80
1203 ± 190 (7.00*)
100
1.0
2109 ± 476 (16.0*)
57
2041 ± 319 (11.0*)
50
TA102
0
197 ± 88 (1.00)
100
193 ± 77 (1.00)
100
0.05
280 ± 4 (1.40)
100
408 ± 24 (2.10*)
100
0.1
289 ± 45 (1.40)
100
459 ± 50 (2.30*)
100
0.2
185 ± 80 (0.90)
100
594 ± 8.0 (3.00*)
100
0.5
347 ± 38 (1.70)
100
680 ± 60 (3.50*)
100
1.0
313 ± 37 (1.70)
100
666 ± 27 (3.40*)
100
Mutagenic index (M.I.): ratio of the his + induced with samples/spontaneous his + in the negative control. S.D.: standard deviation. Positive responses marked in bold when M.I. ≥ 2.
bPercent of cellular survival: <70% was considered toxic dose and it is marked in bold.
*Representation of a significant response (). Negative control was DMSO. Positive controls and their M.I., with and without S9 mix, respectively, were 4NQO (10) and 2AA (6.0) for TA97, 4NQO (15) and 2AA (10) for TA98, AS (16) and 2AA (11) for TA100, and MitC (8.7 and 4.3) for TA102.
