Evaluation of Genotoxic Effects of New Molecules with Possible Trypanocidal Activity for Chagas Disease Treatment
Table 4
PTAL 05-02 induction of mutagenicity and cytotoxicity in Salmonella enterica serovar Typhimurium standard strains, without (−S9) and with (+S9) metabolic activation.
Strains
Dose (g/mL)
−S9
+S9
Mean ± S.D. (M.I.a)
Survivalb
Mean ± S.D. (M.I.a)
Survivalb
TA97
0
87 ± 9.8 (1.00)
100
97 ± 25 (1.00)
100
0.05
89 ± 5.0 (1.03)
90
67 ± 3.5 (0.70)
100
0.1
89 ± 12 (1.03)
100
68 ± 4.0 (0.70)
92
0.5
86 ± 8.5 (0.99)
100
74 ± 0.0 (0.76)
90
1.0
83 ± 1.7 (0.95)
100
69 ± 4.5 (0.71)
80
TA98
0
45 ± 1.5 (1.00)
100
18 ± 6.4 (1.00)
100
0.05
30 ± 2.1 (0.67)
100
18 ± 5.5 (0.96)
95
0.1
38 ± 5.0 (0.83)
98
21 ± 2.0 (1.16)
90
0.5
38 ± 11 (0.84)
100
19 ± 1.7 (1.02)
81
1.0
58 ± 11 (1.28)
94
22 ± 5.2 (1.18)
91
TA100
0
120 ± 23 (1.00)
100
117 ± 19 (1.00)
100
0.05
136 ± 15 (1.13)
100
121 ± 25 (1.04)
79
0.1
102 ± 25 (0.85)
90
106 ± 12 (0.91)
62
0.5
84 ± 10 (0.70)
100
128 ± 18 (1.09)
64
1.0
105 ± 9.8 (0.88)
100
129 ± 13 (1.10)
49
TA1535
0
22 ± 3.2 (1.00)
100
5.3 ± 1.5 (1.00)
100
0.05
21 ± 3.2 (0.94)
100
5.3 ± 0.5 (1.00)
47
0.1
23 ± 2.0 (1.03)
100
5.3 ± 0.5 (1.00)
42
0.5
22 ± 4.0 (1.00)
97
6.6 ± 1.5 (1.25)
40
1.0
26 ± 2.0 (1.15)
93
6.3 ± 2.0 (1.19)
44
TA102
0
254 ± 16 (1.00)
100
265 ± 12 (1.00)
100
0.05
254 ± 31 (1.00)
79
253 ± 19 (0.95)
100
0.1
276 ± 14 (1.09)
84
225 ± 4.0 (0.85)
100
0.5
253 ± 11 (1.00)
79
238 ± 16 (0.90)
100
1.0
261 ± 14 (1.03)
78
256 ± 8.3 (0.97)
100
Mutagenic index (M.I.): ratio of the his + induced with samples/spontaneous his + in the negative control. S.D.: standard deviation. Positive responses marked in bold when M.I. ≥ 2.
bPercent of cellular survival: <70% was considered toxic dose and it is marked in bold.
*Representation of a significant response (). Negative control was DMSO. Positive controls and their M.I., with and without S9 mix, respectively, were 4NQO (7.19) and 2AA (9.55) for TA97, 4NQO (10) and 2AA (3.95) for TA98; AS (13) and 2AA (2.10) for TA100, AS (3.31) and 2AA (6.25) for TA1535, and MitC (9.28 and 6.86) for TA102.
