Immunohistochemical and Molecular Characterization of the Human Periosteum

Table 1

Specific primers used for RT-PCR.

Gene	Annealing temperature	Application of primer	Forward and reverse primers (5^′→3^′)
ALP	50.0°C	Forward	ACG TGG CTA AGA ATG TCA
	51.0°C	Reverse	CTG GTA GGC GAT GTC CTT A
Cbfa-1/Runx2	48.0°C	Forward	TCT TCA CAA ATC CTC CCC
	48.0°C	Reverse	TGG ATT AAA AGG ACT TGG
RANK-L	58.0°C	Forward	CCT ACG CAC AAG GCG AAG ATG C
	58.0°C	Reverse	CGT AGA CCA CGA TGA TGT CGC C
M-CSF	50.0°C	Forward	CTG ACC AGC TCA GAG AGA
	44.0°C	Reverse	CTC ATC AAT GTG CAG GA
SP7-s	55.0°C	Forward	CAG GTT CCC CCA GGA GGA
	58.0°C	Reverse	AGT CCC GCA GAG GGC TAG AG
SP7-l	56.0°C	Forward	TCC TCC CTG CTT GAG GAG GA
	58.0°C	Reverse	AGT CCC GCA GAG GGC TAG AG
Sox-9	57.0°C	Forward	GCC ACG GAG CAG ACG CAC
	55.0°C	Reverse	GCG CCT GCT GCT TGG ACA
TRAP	60.0°C	Forward	CTG GCT GAT GGT GCC ACC CCT G
	60.0°C	Reverse	CTC TCA GGC TGC AGG CTG AGG
CTR	53.0°C	Forward	GCA ATG CTT TCA CTC CTG AGA AA
	53.0°C	Reverse	AGT GCA TCA CGT AAT CAT ATA TC
Cathepsin K	50.0°C	Forward	CCC GAA GGG AAA CAA GCA
	50.0°C	Reverse	GCC TGT ACC TGT ACA GCA
β-Actin	52.0°C	Forward	GGGTCCGTTTGCCAGTCA
	44.0°C	Reverse	ATCCTCGTCGTACACGTA