Table 1: Specific primers used for RT-PCR.

GeneAnnealing temperature Application of primerForward and reverse primers ( )

ALP
50.0°CForwardACG TGG CTA AGA ATG TCA
51.0°CReverseCTG GTA GGC GAT GTC CTT A
Cbfa-1/Runx248.0°CForwardTCT TCA CAA ATC CTC CCC
48.0°CReverseTGG ATT AAA AGG ACT TGG
RANK-L58.0°CForwardCCT ACG CAC AAG GCG AAG ATG C
58.0°CReverseCGT AGA CCA CGA TGA TGT CGC C
M-CSF50.0°CForwardCTG ACC AGC TCA GAG AGA
44.0°CReverseCTC ATC AAT GTG CAG GA
SP7-s55.0°CForwardCAG GTT CCC CCA GGA GGA
58.0°CReverseAGT CCC GCA GAG GGC TAG AG
SP7-l56.0°CForwardTCC TCC CTG CTT GAG GAG GA
58.0°CReverseAGT CCC GCA GAG GGC TAG AG
Sox-957.0°CForwardGCC ACG GAG CAG ACG CAC
55.0°CReverseGCG CCT GCT GCT TGG ACA
TRAP60.0°CForwardCTG GCT GAT GGT GCC ACC CCT G
60.0°CReverseCTC TCA GGC TGC AGG CTG AGG
CTR53.0°CForwardGCA ATG CTT TCA CTC CTG AGA AA
53.0°CReverseAGT GCA TCA CGT AAT CAT ATA TC
Cathepsin K50.0°CForwardCCC GAA GGG AAA CAA GCA
50.0°CReverseGCC TGT ACC TGT ACA GCA
β-Actin52.0°CForwardGGGTCCGTTTGCCAGTCA
44.0°CReverseATCCTCGTCGTACACGTA