Research Article

Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets

Figure 1

Histological observation of layered human endometrial gland-derived mesenchymal stem cells (hEMSC) sheets after a 24 h cultivation on porous membranes. All microphotographs are the hematoxylin and eosin stained cross-sections of the cell sheets ((a) single-layered cell sheet; (b) double-layered cell sheet; (c) triple-layered cell sheet; (d) quadruple-layered cell sheet; (e) quintuple-layered cell sheet; (f) sextuple-layered cell sheet). Enlarged photographs showed chondrocyte-like cells. Independent three experiments were performed and those experiments showed similar results. The representative photographs were shown in the figure.
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(a) Single layer 
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(b) Double layer
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(c) Triple layer
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(d) Quadruple layer
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(e) Quintuple layer
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(f) Sextuple layer