Conformation and Catalytic Properties Studies of Candida rugosa Lip7 via Enantioselective Esterification of Ibuprofen in Organic Solvents and Ionic Liquids

Table 2

Effect of solvents on the enzyme activity and ees via esterification of ibuprofen*.

	Solvent	log𝑃	Enzyme activity (U/g)	ees (%)
1	n-Undecane	6.1	82.67±1.56	49.89±0.02
2	n-Decane	5.6	74.55±1.51	41.57±0.02
3	n-Nonane	5.1	64.27±4.21	35.39±0.01
4	Isooctane	4.7	118.02±5.65	92.01±0.01
5	n-Octane	4.5	79.70±3.13	43.75±0.02
6	n-Heptane	4.0	79.72±4.15	45.51±0.01
7	n-Hexane	3.5	81.24±6.41	42.38±0.02
8	Cyclohexane	3.2	79.01±5.65	43.83±0.01
9	Xylene	2.5	23.84±5.35	8.54±0.04
10	Acetonitrile	−0.33	ND	ND
11	BmimTF2N		60.68±4.64	33.44±0.01
12	BmimPF6		84.95±3.23	53.96±0.02
13	EmimPF6		96.42±5.11	65.84±0.01

∗The reactions were performed at 50°C, 200 rpm for 12 h. 0.1 g CRL7 was added to 5 mL solvent (from log𝑃=6.1 to log𝑃=−0.33) containing 0.01 mmol ibuprofen, 1.0 mmol isooctanol. The data were measured in triplicate and expressed in mean ± standard deviation (SD); ND indicates not determined.