Generation of Mature N^α-Terminal Acetylated Thymosin α1 by Cleavage of Recombinant Prothymosin α

Figure 3

RP-HPLC analysis of N^α-acetylated Tα1. (a) Sample purified by PoRos R50 column was analyzed by RP-HPLC and eluted as two peaks, I and II corresponding to nonacetylated Tα1 (14.846 min) and N^α-acetylated Tα1 (15.396 min). (b–d) RP-HPLC analysis of samples eluted from SP column with (b) 0.2 M NaCl, (c) 0.3 M NaCl, and (d) 0.5 M NaCl. N^α-acetylated Tα1 was washed out in 0.2 M NaCl fraction (2, peak II), while nonacetylated Tα1 was washed out in 0.5 M NaCl fraction (4, peak I).

(a)

(b)

(c)

(d)