Nucleotide Sequencing and Identification of Some Wild Mushrooms

Table 1

Primers designed for different mushroom sample amplification.

Sl. no.	Primer name	Sequence (5′ to 3′)	Size (bp)	Melting temp. (°C)	Amplicon size (bp)
1	Len-F	TGGTTACTCTACAAACACTT	20	54
2	Len-R	CCTGCTAATGTATTTCAGAAG	21	58	331
3	Vol-F	GATCATTACAGAATCGAACGC	21	60
4	Vol-R	CTGGGCTTGAGGATTCGATG	20	62	290
5	Rus-F	AGTGCTCTCACATACAAATATC	22	60
6	Rus-R	GTTGAGGATGTTCACGACACTC	22	66	290
7	Ter-F	GCAACGGCACTCTATCGCTGA	21	66
8	Ter-R	CTCCTCAGATCACCAAGGAG	20	62	321
9	Ast-F	CTCCAACTTCATCAAGAAGGT	21	60
10	Ast-R	GATCTTGTAGACATCCTGGAGAG	23	68	230
11	Ama-F	TCTTGGCTCTCGCATCGATG	20	62
12	Ama-R	CCAGCAATGACTCCCAATATC	21	62	200