VEGF Promotes Proliferation of Human Glioblastoma Multiforme Stem-Like Cells through VEGF Receptor 2
Figure 2
Effect of VEGF on GBSCs proliferation. GBSCs were treated with indicated concentration of VEGF in the presence or absence of VEGFR inhibitors, followed by Neural Colony-Forming Cell Assay and cell viability analysis using ATP assay. (a)–(c) dose effect of VEGF on GBSCs proliferation. (d)–(f) effect of VEGFR2 inhibition on GBSCs proliferation. (g)–(i) effect of VEGFR1 inhibition on GBSCs proliferation. Panels (a), (d), and (g) representative images of neurospheres formed in neurosphere formation assays. Panels (b), (e), and (h) GBSC proliferation was tested using a Neural Colony-Forming Cell Assay; all data are represented as the percent of wells with neurospheres. Panels (c), (f), and (i) GBSCs proliferation was tested using ATP assay; all data are represented as a percentage of the normoxia control. The statistical significance in comparisons between the different groups is indicated using the capped lines (*, **).
