VEGF Promotes Proliferation of Human Glioblastoma Multiforme Stem-Like Cells through VEGF Receptor 2
Figure 3
Effect of VEGF on VEGFR expression. GBSCs were treated with the indicated concentration of VEGF in the presence or absence of the VEGFR inhibitor or antibody. The levels of total VEGFR1 and VEGFR2 protein and the levels of phosphorylated VEGFR1 and VEGFR2 were determined by Western blot analysis and expressed relative to β-actin protein level. Phosphorylated VEGFR1 and VEGFR2 protein levels were normalized to total VEGFR1 and VEGFR2, respectively. Values represent the mean ± SD of three separate experiments. The statistical significance in comparisons between the different groups is indicated using the capped lines (N = normoxia, H = hypoxia;*, **).
