A New gcrR-Deficient Streptococcus mutans Mutant for Replacement Therapy of Dental Caries

Table 1

Primers used in identification of the combination.

Primer name	Primer sequences (5′–3′)	Tm (°C)	Applications	Amplicon size
P1	AAAGCCCAGCAGCATCAC	56	Verifying the correct mutant	1434 bp
P2	TGCCGAGAAAGTATCCATCA
RT-gcrRF	CAGCTCGGTTATGAACATCT	52	RT-PCR of gcrR	207 bp
RT-gcrRR	TTATCACTGCTCGTGACTCTAT