Evaluation and Optimization of Downstream Process Parameters for Extraction of Betulinic Acid from the Bark of Ziziphus jujubae L.

Table 2

Design matrix for the optimization of extraction parameters for maximum recovery of betulinic acid as response.

Run	Type	Factors			BA yield (% w/w)
		pH	Time (min)	Temp. (°C)	Observed	Predicted
1	Center	6.50	3.50	45.00	0.21	0.24
2	Center	6.50	3.50	45.00	0.21	0.24
7	Center	6.50	3.50	45.00	0.21	0.24
8	Center	6.50	3.50	45.00	0.21	0.24
16	Center	6.50	3.50	45.00	0.35	0.37
19	Center	6.50	3.50	45.00	0.21	0.24
3	Axial	6.50	3.50	70.23	0.4	0.44
6	Axial	0.61	3.50	45.00	0.06	0.07
15	Axial	12.39	3.50	45.00	0.1	0.12
17	Axial	6.50	3.50	19.77	0.08	0.09
18	Axial	6.50	6.02	45.00	0.21	0.24
20	Axial	6.50	0.98	45.00	0.07	0.09
4	Factorial	10.00	5.00	60.00	0.25	0.26
5	Factorial	3.00	5.00	30.00	0.06	0.09
9	Factorial	10.00	5.00	30.00	0.12	0.14
10	Factorial	3.00	2.00	60.00	0.24	0.26
11	Factorial	10.00	2.00	60.00	0.29	0.31
12	Factorial	3.00	5.00	60.00	0.33	0.34
13	Factorial	3.00	2.00	30.00	0.04	0.06
14	Factorial	10.00	2.00	30.00	0.05	0.04