In Astrocytes the Accumulation of the Immunity-Related GTPases Irga6 and Irgb6 at the Vacuole of Toxoplasma gondii Is Dependent on the Parasite Virulence

Figure 2

Accumulation of Irga6 and Irgb6 at the PV of different T. gondii strains in astrocytes. Astrocytes were prestimulated with 100 U/mL IFNγ, pulse-infected with different T. gondii strains (MOI: 10) for the indicated time points, and stained for Irga6 ((a)–(d)) and Irgb6 ((e)–(h)). PVs were identified via nucleus staining of tachyzoites with DAPI. ((a)–(d)) Counted Irga6⁺ vacuoles were expressed as percent of all PVs. (a): ME49; (b): NTE; (c): BK; (d): RH. ((e)–(h)) Counted Irgb6⁺ vacuoles were expressed as percent of all PVs. (e): ME49; (f): NTE; (g): BK; (h): RH. The data represent means of five independent infection experiments in duplicates.

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